Kanamycin 6'-acetate and ribostamycin 6'-acetate, enzymatically inactivated products by Pseudomonas aeruginosa.

The enzyme solution (S-100) was prepared by the procedure described in a previous paper.l) The inactivation was carried out at 37°C for 3 hours in the following reaction mixture : 100 mg (0.2 mmoles) of kanamycin or 91mg (0.2mmoles) of ribostamycin in 25ml of water, 4,842mg (8.0mmoles) of disodium ATP trihydrate in 100 ml of 0.8 % sodium bicarbonate, 25 mg (0.03 mmoles) of coenzyme A in 50ml of water, 25ml of the enzyme solution (20mg/ml protein),